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Contrasting effects of historical contingency on phenotypic and genomic trajectories during a two-step evolution experiment with bacteria ArchiMer
Plucain, Jessica; Suau, Antonia; Cruveiller, Stephane; Medigue, Claudine; Schneider, Dominique; Le Gac, Mickael.
Background The impact of historical contingency, i.e. the past evolutionary history of a population, on further adaptation is mostly unknown at both the phenotypic and genomic levels. We addressed this question using a two-step evolution experiment. First, replicate populations of Escherichia coli were propagated in four different environmental conditions for 1000 generations. Then, all replicate populations were transferred and propagated for further 1000 generations to a single new environment. Results Using this two-step experimental evolution strategy, we investigated, at both the phenotypic and genomic levels, whether and how adaptation in the initial historical environments impacted evolutionary trajectories in a new environment. We showed that...
Tipo: Text Palavras-chave: Experimental evolution; Escherichia coli; Adaptation; Historical contingency; Epistasis.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00333/44447/44118.pdf
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Evolutionary history and genetic parallelism affect correlated responses to evolution ArchiMer
Le Gac, Mickael; Cooper, Tim F.; Cruveiller, Stephane; Edigue, Claudine M.; Schneider, Dominique.
We investigated the relationship between genomic and phenotypic evolution among replicate populations of Escherichia coli evolved for 1000 generations in four different environments. By resequencing evolved genomes, we identified parallel changes in genes encoding transcription regulators within and between environments. Depending on both the environment and the altered gene, genetic parallelism at the gene level involved mutations that affected identical codons, protein domains or were widely distributed across the gene. Evolved clones were characterized by parallel phenotypic changes in their respective evolution environments but also in the three alternative environments. Phenotypic parallelism was high for clones that evolved in the same environment,...
Tipo: Text Palavras-chave: Adaptation; Epistasis; Experimental evolution; Genome sequencing; Parallelism.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00148/25928/24096.pdf
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Springtime changes in snow chemistry lead to new insights into mercury methylation in the Arctic ArchiMer
Larose, Catheririe; Dommergue, Aurelien; De Angelis, Martine; Cossa, Daniel; Averty, Bernard; Marusczak, Nicolas; Soumis, Nicolas; Schneider, Dominique; Ferrari, Christophe.
Seasonal snow is an active media and an important climate factor that governs nutrient transfer in Arctic ecosystems. Since the snow stores and transforms nutrients and contaminants, it is of crucial importance to gain a better understanding of the dynamics of contaminant cycling within the snowpack and its subsequent release to catchments via meltwater. Over the course of a two-month field study in the spring of 2008, we collected snow and meltwater samples from a seasonal snowpack in Ny-Alesund, Norway (78 degrees 56'N, 11 degrees 52'E), which were analyzed for major inorganic ions and some organic acids, as well as total, dissolved, bioavailable mercury (THg, DHg, BioHg, respectively) and monomethylmercury (MMHg) species. We observe a seasonal gradient...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12904/9880.pdf
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